L'\u00e9quipe Xenome recrute r\u00e9guli\u00e8rement des personnes de tout niveau (stagiaires, \u00e9tudiants en th\u00e8se, post-doctorants). Nous recherchons des personnes passionn\u00e9es et investies, qui auront l'opportunit\u00e9 de travailler sur des sujets qui touchent \u00e0 la biologie synth\u00e9tique, la diversification des acides nucl\u00e9iques et la biodiversit\u00e9 des organismes. Les candidats int\u00e9greront un environnement pluridisciplinaire et dynamique.<\/p>\n\n\n\n
Post doc<\/strong><\/p>\n\n\n\n Laboratoires : LiSSB (Laboratoire de biologie synth\u00e9tique et syst\u00e9mique), Equipe X\u00e9nome dirig\u00e9e par Val\u00e9rie P\u00e9zo et LabGEM (Laboratoire d'Analyses Bioinformatiques pour la Genomique et le Metabolisme) : Rapha\u00ebl Meheust.<\/p>\n\n\n\n Le projet BEMOD, financ\u00e9 par l'Agence Nationale de la Recherche (ANR), vise \u00e0\nidentifier les prot\u00e9ines et \u00e0 comprendre les m\u00e9canismes impliqu\u00e9s dans la propagation des bact\u00e9riophages \u00e0 ADN modifi\u00e9. Certains virus \u00e0 ADN infectant des h\u00f4tes, tels que les prot\u00e9obact\u00e9ries, les cyanobact\u00e9ries et les les actinobact\u00e9ries, pr\u00e9sentent une substitution totale de l'ad\u00e9nine par l'aminoad\u00e9nine (Z), formant ainsi trois liaisons hydrog\u00e8ne dans les paires Z:T. La voie de biosynth\u00e8se de l'amino d\u00e9soxyad\u00e9nosine triphosphate (dZTP) a \u00e9t\u00e9 \u00e9lucid\u00e9e pour deux bact\u00e9riophages contenant des g\u00e9nomes ZTGC, et nous avons montr\u00e9 que les ADN polym\u00e9rases de ces phages discriminent en faveur de l'incorporation de l'aminoad\u00e9nine et contre celle de l'ad\u00e9nine canonique.\nCes bact\u00e9riophages codent \u00e9galement pour des enzymes modifiant le m\u00e9tabolisme des nucl\u00e9otides qui favorisent probablement la synth\u00e8se de leur g\u00e9nome substitu\u00e9.<\/p>\n\n\n\n Mission:<\/p>\n\n\n\n Le premier objectif de ce projet postdoctoral est de rechercher de nouveaux g\u00e9nomes de bact\u00e9riophages \u00e0 ADN-Z dans des bases de donn\u00e9es m\u00e9tag\u00e9nomiques afin d'\u00e9tudier leur distribution environnementale. Nous explorerons ensuite le contenu g\u00e9nique des phages \u00e0 ADN-Z, en nous concentrant sur les g\u00e8nes codant pour les enzymes impliqu\u00e9es dans la propagation de l'ADN-Z. Des r\u00e9sultats pr\u00e9liminaires ont identifi\u00e9 diff\u00e9rents types d'enzymes impliqu\u00e9es dans la propagation des g\u00e9nomes Z : des enzymes impliqu\u00e9es dans la biosynth\u00e8se des nucl\u00e9otides, les voies de r\u00e9cup\u00e9ration, les enzymes de la machinerie de r\u00e9plication et les ADN polym\u00e9rases. Cette partie sera r\u00e9alis\u00e9e avec Rapha\u00ebl M\u00e9heust, un expert en g\u00e9nomique comparative. Le second objectif sera de caract\u00e9riser fonctionnellement les candidats les plus int\u00e9ressants. Des r\u00e9sultats pr\u00e9liminaires ont r\u00e9v\u00e9l\u00e9 des fonctions sp\u00e9cifiques et inattendues de certaines enzymes adapt\u00e9es aux sp\u00e9cificit\u00e9s de ces bact\u00e9riophages.<\/p>\n\n\n\n Comp\u00e9tences techniques et comportementales :<\/p>\n\n\n\n Doctorat en biologie mol\u00e9culaire, microbiologie, biochimie Comment appliquer :<\/p>\n\n\n\n Veuillez envoyer votre CV et une lettre de motivation d\u00e9crivant vos int\u00e9r\u00eats de recherche, vos comp\u00e9tences et votre motivation \u00e0 vpezo@genoscope.cns.fr et raphael.meheust@genoscope.cns.fr<\/p>\n\n\n\n Date de prise de fonction : fin 2025 \/ d\u00e9but 2026 Salaire : fonction de l'exp\u00e9rience et qualifications (grilles CEA).<\/p>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":" Xenome team is often looking for enthusiastic and talented trainees, technicians, PhD students and post-docs who are interested in synthetic biology, nucleic acid diversification and organisms biodiversity. Candidates will have the opportunity to work in a diverse, interdisciplinary and dynamic environment. Postdoctoral position Exploring the enzymes involved in the propagation of bacteriophages with modified DNA Genoscope \u2013 CEA, Evry (France) Laboratories: LiSSB (Laboratory of Systems and Synthetic Biology) Xenome Team headed by Val\u00e9rie P\u00e9zo and LabGEM (Laboratory of Bioinformatics for Genomics and Metabolism) Rapha\u00ebl M\u00e9heust The BEMOD project, funded by the French National Research Agency (ANR), aims to identify proteins and understand the mechanisms involved in the propagation of bacteriophages with modified DNA. We focused on bacteriophages containing a total substitution of their genomic adenine (A) with aminoadenine (Z) resulting in a Z:T base pairing with 3 hydrogen bonds. The amino deoxyadenosine triphosphate (dZTP) biosynthetic pathway has been elucidated for two bacteriophages containing ZTGC genomes and we have shown that the DNA polymerases of these phages discriminate in favor of aminoadenine incorporation and against canonical adenine. These bacteriophages also encode nucleotide metabolism modifying enzymes that likely promote the synthesis of their substituted genome. Mission: The first objective of this postdoc project is to search for new bacteriophage genomes with Z-DNA in metagenomic databases in order to study their environmental distribution. We will next explore the gene content of Z-DNA phages with a focus on genes encoding enzymes involved in the Z-DNA propagation. Preliminary results identified various types of enzymes implicated in Z genomes propagation: enzymes implicated in the nucleotide biosynthesis, salvage pathways, enzymes of replication machinery and DNA polymerases. This part will be done with Raphael M\u00e9heust, an expert in comparative genomics. The second objective will be to functionally characterize the most interesting candidates. Preliminary results revealed specific and unexpected functions of certain enzymes adapted to these bacteriophage specificities. Required qualifications: \u2022 A PhD in molecular biology, microbiology, biochemistry or related fields\u2022 Expertise in biochemistry, molecular cloning DNA, enzymology or DNA replication systems\u2022 Strong interest in protein evolution or structural biology\u2022 Ability to work independently and collaboratively in an interdisciplinary team How to apply: Please send your CV and a cover letter describing your research interests, skills and motivation to vpezo@genoscope.cns.fr and raphael.meheust@genoscope.cns.fr Starting date: end of 2025 or beginning of 2026Duration: 2 years Salary: According to experience and qualifications (CEA salary scale)<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-560","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/xenome.genoscope.cns.fr\/fr\/wp-json\/wp\/v2\/pages\/560","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/xenome.genoscope.cns.fr\/fr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/xenome.genoscope.cns.fr\/fr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/xenome.genoscope.cns.fr\/fr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/xenome.genoscope.cns.fr\/fr\/wp-json\/wp\/v2\/comments?post=560"}],"version-history":[{"count":12,"href":"https:\/\/xenome.genoscope.cns.fr\/fr\/wp-json\/wp\/v2\/pages\/560\/revisions"}],"predecessor-version":[{"id":600,"href":"https:\/\/xenome.genoscope.cns.fr\/fr\/wp-json\/wp\/v2\/pages\/560\/revisions\/600"}],"wp:attachment":[{"href":"https:\/\/xenome.genoscope.cns.fr\/fr\/wp-json\/wp\/v2\/media?parent=560"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Exploration des enzymes impliqu\u00e9es dans la propagation des bact\u00e9riophages \u00e0 ADN modifi\u00e9
Genoscope \u2013 CEA, Evry (France)<\/strong><\/p>\n\n\n\n
Expertise en biochimie, clonage mol\u00e9culaire d'ADN, enzymologie ou syst\u00e8mes de r\u00e9plication d'ADN
Fort int\u00e9r\u00eat pour l'\u00e9volution des prot\u00e9ines ou la biologie structurale
Capacit\u00e9 \u00e0 travailler de mani\u00e8re ind\u00e9pendante et en collaboration au sein d'une \u00e9quipe interdisciplinaire.<\/p>\n\n\n\n
Dur\u00e9e : 2 ans<\/p>\n\n\n\n